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Differential down‐regulation of protein kinase C subspecies in KM3 cells
Author(s) -
Ase Katsuhiko,
Berry Nicola,
Kikkawa Ushio,
Kishimoto Akira,
Nishizuka Yasutomi
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80064-4
Subject(s) - subspecies , differential (mechanical device) , microbiology and biotechnology , protein kinase a , chemistry , kinase , biology , physics , zoology , thermodynamics
The down‐regulation of protein kinase C (PKC) subspecies in KM3 cells (a pre‐B, pre‐T cell line) has been examined. The PKC from KM3 cells was resolved into two subspecies, type II (mainly βII) and type III (α), upon hydroxyapatite column chromatography. Biochemical and immunocytochemical analysis revealed that, when these cells were treated with 12‐ O ‐tetradecanoylphorbol 13‐acetate (TPA), the time course of down‐regulation of the PKC subspecies was different; type II PKC was translocated and depleted from the cell more quickly than type III enzyme. The results suggest that each PKC subspecies plays a different role in the cellular response to TPA and probably to other external stimuli.