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Production of a specific antibody against pyruvate kinase type M2 using a synthetic peptide
Author(s) -
Oude Weernink Paschal A.,
Rijksen Gert,
Staal Gerard E.J.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80063-2
Subject(s) - pyruvate kinase , pkm2 , isozyme , biochemistry , antiserum , antibody , peptide , chemistry , peptide sequence , enzyme , microbiology and biotechnology , biology , glycolysis , gene , immunology
The pyruvate kinase isozymes M1 and M2 are structurally and immunologically closely related. To obtain an antibody which discriminates between these two forms, a synthetic tetradecapeptide with a sequence specific for pyruvate kinase type M2 from rats was constructed. Antisera from rabbits, immunized with this peptide, reacted specifically with the M2‐type holoenzyme of both rat and human origin, and did not cross‐react with the M1‐type isozyme. This was established by immunoblot analysis, both under dissociating and non‐dissociating conditions.