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Circular dichroism and fluorescence of polyethylene glycol‐subtilisin in organic solvents
Author(s) -
Pasta P.,
Riva S.,
Carrea G.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80049-8
Subject(s) - subtilisin , circular dichroism , chemistry , acetonitrile , polyethylene glycol , fluorescence , covalent bond , photochemistry , organic chemistry , enzyme , stereochemistry , physics , quantum mechanics
Subtilisin Carlsberg has been made soluble in organic solvents such as dioxane and acetonitrile by covalent linking to polyethylene glycol. Far‐ultraviolet circular dichroism and intrinsic protein fluorescence have shown that subtilisin dissolved in dioxane, in which the enzyme is active and highly stable, maintains the native secondary structure as well as the native microenvironment for tyrosyl residues. In acetonitrile subtilisin undergoes conformational changes that cause enzyme inactivation and precipitation.