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Diguanosine 5′ ,5‴‐ P 1 , P 4 ‐tetraphosphate and other purine nucleotides inhibit endoribonuclease VI from Artemia
Author(s) -
Grau Pilar P.,
Heredia Claudio F.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80040-1
Subject(s) - nucleotide , ribonuclease , endoribonuclease , purine , enzyme , chemistry , biochemistry , uridine , stereochemistry , rnase p , rna , gene
The activity of the endoribonuclease VI from Artemia is sensitive to several purine nucleotides. The enzyme is non‐competitively inhibited by diguanosine tetraphosphate ( K i = 75 μM), a nucleotide abundant in Artemia encysted gastrulae and located in the same particulate fraction as the gastrular ribonuclease. Diguanosine triphosphate and diadenosine tetraphosphate are less efficient inhibitors ( K i ⋍200 μM). The ribonuclease is non‐competitively inhibited by 5′‐AMP ( K i = 10 μM) and 5′‐GMP ( K i = 50 μM) but is insensitive to the corresponding 5′‐phosphates of cytosine and uridine. Other purine mononucleotides inhibit the enzyme activity less efficiently. The modulation of the enzyme activity by these nucleotides is discussed in relation with the changes in ribonuclease activity during early development of Artemia .