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Genotyping of human class I alcohol dehydrogenase Analysis of enzymatically amplified DNA with allele‐specific oligonucleotides
Author(s) -
Gennari Katharina,
Wermuth Bendicht,
Muellener Daniel,
Ehrig Torsten,
von Wartburg Jean-Pierre
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80021-8
Subject(s) - alcohol dehydrogenase , genotyping , isozyme , oligonucleotide , genomic dna , polymerase chain reaction , alcohol , biology , genetics , allele , microbiology and biotechnology , adh1b , biochemistry , dna , enzyme , chemistry , gene , genotype , dehydrogenase , branched chain alpha keto acid dehydrogenase complex
Large inter‐individual differences are noted in the susceptibility to alcohol‐related problems. Part of this variation may be due to the different isoenzyme patterns of the alcohol‐metabolizing enzymes and, consequently, different pharmacokinetics of alcohol degradation. We have used the polymerase chain reaction and oligonucleotide hybridization to amplify and analyze class I alcohol dehydrogenase isoenzyme‐specific genomic DNA. The method unambiguously distinguishes between different allelic variants and thus provides a new means of elucidating the alcohol dehydrogenase isoenzyme pattern of humans.