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Cloning and sequencing of cDNA that encodes goat growth hormone
Author(s) -
Yamano Yoshiaki,
Oyabayashi Kazuo,
Okuno Mitsuhiro,
Yato Miki,
Kioka Noriyuki,
Manabe Eichi,
Hashi Hidetaka,
Sakai Hiroshi,
Komano Tohru,
Utsumi Kyozo,
Iritani Akira
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80020-6
Subject(s) - complementary dna , cdna library , homology (biology) , biology , microbiology and biotechnology , gene , cloning (programming) , coding region , peptide sequence , nucleic acid sequence , untranslated region , amino acid , molecular cloning , southern blot , genetics , messenger rna , computer science , programming language
The cDNA that encodes goat growth hormone (gGH) was isolated from a goat pituitary cDNA library. The cDNA, about 880 base pairs long, had a coding sequence, 5′‐ and 3′‐untranslated regions and a poly(A) chain. The cDNA could encode a polypeptide of 217 amino acids. The amino acid sequence homology between gGH and the sequences of bovine GH, rat GH and human GH was 99, 83 and 66%, respectively. By Northern blot hybridization, we found that the possible gGH gene is transcribed in the goat pituitary.