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Isolation of the intact photosystem I reaction center core containing P700 and iron‐sulfur center F X
Author(s) -
Golbeck John H.,
Parrett Kevin G.,
Mehari Tetemke,
Jones Karen L.,
Brand Jerry J.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80013-9
Subject(s) - p700 , photosynthetic reaction centre , photosystem i , chemistry , ultracentrifuge , sulfide , sulfur , photochemistry , photosystem ii , electron transfer , photosynthesis , biochemistry , organic chemistry
The photosystem I reaction center core containing P700 and iron‐sulfur center F X has been isolated from a Synechococcus photosystem I particle with 6.8 M urea at pH 10.0 followed by sucrose density ultracentrifugation. The reaction center core has retained > 90% of F X and 100% of P700 (determined by optical spectroscopy) but is totally devoid of iron‐sulfur centers F A and F B (determined by optical and ESR spectroscopy). SDS‐PAGE indicates the retention of the 57 kDa reaction center polypeptide(s) but the total absence of the 16.4 and 8.1 kDa polypeptides. The loss of F A and F B is further reflected in the decline of acid‐labile sulfide from 11.8 ± 0.4 S 2− /P700 in the control particle to 4.6 ± 0.3 S 2− /P700 in the reaction center core. This preparation represents the first isolation of an intact reaction center core incorporating the components P700 and F X but totally lacking F A and F B .