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Bioenergetics of dihydrostreptomycin transport by Escherichia coli
Author(s) -
Goss Stephen R.,
Spicer Alan B.,
Nichols Wright W.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80008-5
Subject(s) - dihydrostreptomycin , escherichia coli , bioenergetics , chemistry , biophysics , cytoplasm , atp hydrolysis , apyrase , membrane potential , membrane transport , biochemistry , membrane , biology , enzyme , antibiotics , streptomycin , atpase , mitochondrion , gene
Previous demonstrations of the irreversibility of dihydrostreptomycin transport across the cytoplasmic membrane of Escherichia coli were not due to decreases in the magnitude of the cytoplasmic membrane potential (Δψ). Irreversibility was probably not due to ATP hydrolysis being coupled to transport, because the rate of energy‐dependent dihydrostreptomycin uptake was unaffected by 10‐fold reduction in the cellular ATP level.