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Opposite effects of adenosine on two types of cAMP‐induced gene expression in Dictyostelium indicate the involvement of at least two different intracellular pathways for the transduction of cAMP signals
Author(s) -
Spek Wouter,
van Drunen Kees,
van Eijk Ronald,
Schaap Pauline
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80005-x
Subject(s) - adenosine , gene expression , signal transduction , biology , gene , extracellular , intracellular , transduction (biophysics) , dictyostelium , microbiology and biotechnology , regulation of gene expression , cyclic adenosine monophosphate , biochemistry , receptor
Adenosine promotes the cAMP‐induced increase of mRNAs, probed with the cDNAs D11 and D14, which are preferentially expressed in prestalk cells, while it inhibits cAMP‐induced prespore gene expression. Half‐maximal inhibition of prespore gene expression occurs at about 300 μM, while prestalk stimulation by adenosine occurs at about 100‐fold lower concentrations and requires the presence of cAMP. These results indicate that adenosine interferes with the transduction of cAMP to gene expression and suggest the involvement of two different adenosine target sites. Our data furthermore indicate that the transduction of extracellular cAMP to prespore gene or prestalk gene expression occurs via divergent pathways.