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The enhanced 32 P labeling of CDP‐diacylglycerol in c‐ myc gene expressed human kidney cancer cells
Author(s) -
Kubota Yoshinobu,
Shuin Taro,
Yao Masahiro,
Inoue Hiroko,
Yoshioka Tohru
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81577-6
Subject(s) - diacylglycerol kinase , cell culture , pi , human kidney , microbiology and biotechnology , gene , gene expression , phosphatidylinositol , cell , kidney , biology , cancer cell , cancer , chemistry , biochemistry , enzyme , genetics , protein kinase c , signal transduction
Correlation between c‐ myc gene expression and phosphatidylinositol (PI) metabolism was studied using seven human kidney cancer cell lines. We found that the exceptional incorporation of 32 P into CDP‐diacyl‐glycerol (CDP‐DG) was observed in only two cell lines, in which c‐ myc expression was increased among seven cell lines tested. In these two cell lines, PI was also labeled. The other five cell lines indicated the accumulation of radioactive PI, whereas CDP‐ DG was unlabeled.