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Adenosine decreases intracellular free calcium concentrations in cultured vascular smooth muscle cells from rat aorta
Author(s) -
Kai Hisashi,
Kanaide Hideo,
Matsumoto Takahiro,
Shogakiuchi Yoshito,
Nakamura Motoomi
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81568-5
Subject(s) - adenosine , vascular smooth muscle , vasodilation , chemistry , intracellular , calcium , biophysics , medicine , depolarization , endocrinology , calcium in biology , aorta , smooth muscle , biochemistry , biology
Using an intracellularly trapped dye, quin 2, effects of adenosine on intracellular free calcium concentrations ([Ca 2+ ] i ) were recorded, microfluorometrically, using rat aortic medial vascular smooth muscle cells (VSMCs) in primary culture. Regardless of whether cells were at rest (in 5 mM K + ), at K + ‐depolarization (in 55 mM K + ) or at Ca 2+ depletion (in Ca 2+ ‐free media), adenosine induced a rapid reduction of [Ca 2+ ] i , following which there was a gradual increase to pre‐exposure levels, in cells at rest and in the case of Ca 2+ depletion. Only when the cells were depolarized (55 mM K + ) did adenosine induce a new steady [Ca 2+ ] i level, lower than the pre‐exposure value. These findings indicate that decrease in [Ca 2+ ] i by adenosine is one possible mechanism involved in the adenosine‐mediated vasodilatation, and that adenosine decreases [Ca 2+ ] i by direct extrusion, by sequestration, or by inhibiting the influx of Ca 2+ into VSMCs.