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Adenylate ratios in the cytosol, chloroplasts and mitochondria of barley leaf protoplasts during photosynthesis at different carbon dioxide concentrations
Author(s) -
Gardeström Per
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81567-3
Subject(s) - chloroplast , photosynthesis , hordeum vulgare , cytosol , protoplast , darkness , adenylate kinase , carbon dioxide , botany , mitochondrion , biology , biochemistry , chemistry , biophysics , poaceae , enzyme , ecology , gene
Barley ( Hordeum vulgare ) protoplasts were incubated in darkness and in the light at saturating and limiting CO 2 concentrations. The protoplasts were fractioned by a membrane filtration technique which allows quenching of the metabolism by acidification within about 0.1 s and the ATP/ADP ratios in the cytasol, chloroplasts and mitochondria were determined. It is concluded that the cytosolic ATP/ADP ratio is considerably higher during photosynthesis at limiting CO 2 (which is the normal situation for a C 3 plant in air) compared to photosynthesis at saturating CO 2 or darkness.