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Vapor‐phase modification of sulfhydryl groups in proteins
Author(s) -
Amons R.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81558-2
Subject(s) - cysteine , tributylphosphine , chemistry , disulfide bond , derivative (finance) , sequence (biology) , chromatography , phase (matter) , biochemistry , organic chemistry , enzyme , financial economics , economics , catalysis
Proteins and peptides are readily and specifically modified at their sulfhydryl groups by the vapors of a mixture of 4‐vinylpyridine and tributylphosphine. The phenylthiohydantoin derivative of S ‐β‐(4‐pyridylethyl) cysteine formed during sequence analysis is easily detectable in current identification systems.