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Induction of cyclo‐oxygenase by interleukin‐1 in rheumatoid synovial cells
Author(s) -
O'Neill L.A.J.,
Barrett M.L.,
Lewis G.P.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81551-x
Subject(s) - arachidonic acid , prostaglandin e2 , chemistry , aspirin , interleukin , prostaglandin e , prostaglandin , immunology , biochemistry , pharmacology , medicine , endocrinology , cytokine , enzyme
The ability of interleukin‐1 (IL‐1) to stimulate prostaglandin E 2 (PGE 2 production by human rheumatoid adherent synovial cells was found to be time‐dependent and sensitive to protein synthesis inhibitors. Cells incubated with exogenous arachidonic acid (10 μM) showed no increase in PGE 2 production. However, with IL‐1 (2.5) and exogenous arachidonic acid there was a marked increase, with levels reaching twice that for cells incubated with IL‐1 alone. Aspirin pre‐treatment studies and the use of [ acetyl ‐ 14 C]aspirin showed that IL‐1 increased PGE 2 production through the induction of cyclo‐oxygenase.