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Competition between tetracycline and tRNA at both P and A sites of the ribosome of Escherichia coli
Author(s) -
Epe Bernd,
Woolley Paul,
Hornig Horst
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81539-9
Subject(s) - tetracycline , transfer rna , escherichia coli , ribosome , chemistry , a site , binding site , ribosomal binding site , competition (biology) , shine dalgarno sequence , biology , biochemistry , antibiotics , rna , ecology , gene
Fluorescence anisotropy studies performed on 6‐demethylchlortetracycline, binding to the ribosome of E. coli in competition with tRNA at the P site or at both P and A sites, have provided a quantitative assessment in situ of the interaction of this antibiotic with the A site and have demonstrated that there is also an interaction between tetracycline and the P site.