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Oxidation‐reduction midpoint potentials of the molybdenum center in spinach NADH:nitrate reductase
Author(s) -
Barber Michael J.,
Notton Brian A.,
Solomonson Larry P.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81524-7
Subject(s) - molybdenum , nitrate reductase , chemistry , nitrate , center (category theory) , spinach , inorganic chemistry , biochemistry , organic chemistry , crystallography
Oxidation‐reduction midpoint potentials for the molybdenum center in assimilatory NADH:nitrate reductase isolated from spinach ( Spinacia oleracea ) have been determined at pH 7.0 in the presence of dye mediators using EPR spectroscopy to monitor formation of Mo(V). Values for the Mo(VI)/Mo(V) and Mo(V)/Mo(IV) couples were determined to be −8 and −42 mV, respectively.