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ATP release from pure cholinergic synaptosomes is not blocked by tetanus toxin
Author(s) -
Rabasseda Xavier,
Solsona Carles,
Marsal Jordi,
Egea Gustau,
Bizzini Bernard
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81518-1
Subject(s) - tetanus , toxin , cholinergic , chemistry , pharmacology , biochemistry , neuroscience , biology , immunology , vaccination
Tetanus toxin (TeTx) is a neurotransmission impairing toxin that acts on several neurotransmitter systems. TeTx also inhibits the K + ‐induced release of acetylcholine (ACh) from synaptosomes isolated from the electric organ of Torpedo . Neither the membrane potential and depolarization, nor the depolarization‐induced calcium uptake into cholinergic nerve terminals is modified after TeTx poisoning. On the other hand, it is known that, when cholinergic nerve terminals are stimulated, there is a release of ATP associated with the release of ACh. We have explored the action of TeTx on this co‐release, and have found that there is no action of TeTx on the nucleotide release. Thus, TeTx blocks ACh release without modifying ATP release.