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dam methylase from E. coli
Author(s) -
Kriebardis Anastasios,
Guschlbauer Wilhelm
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81509-0
Subject(s) - palindromic sequence , enzyme , methyltransferase , dna , biochemistry , sequence (biology) , chemistry , spectroscopy , palindrome , biology , stereochemistry , methylation , physics , gene , genome , quantum mechanics
The enzyme dam methylase which recognizes and methylates the adenine in the palindromic sequence GATC in DNA was isolated and the secondary structure was determined by CD spectroscopy and various predicting methods from the amino acid sequence. The interaction of dam methylase with S ‐adenosylmethionine was studied by CD spectroscopy indicating a decrease of the percentage of α‐helix as the amount of S ‐adenosylmethionine bound to the enzyme was increased.