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The only inositol tetrakisphosphate detectable in avian erythrocytes is the isomer lacking phosphate at position 3: a NMR study
Author(s) -
Mayr Georg W.,
Dietrich Wolfgang
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81505-3
Subject(s) - inositol , polyphosphate , inositol phosphate , biochemistry , chemistry , effector , phosphate , phosphorylation , stereochemistry , receptor
Avian red blood cells contain a millimolar amount of inositol polyphosphate which plays a role as an allosteric effector of hemoglobin. We confirmed the structure of this substance by NMR techniques as purely myo ‐inositol 1,3,4,5,6‐pentakisphosphate. Based on present knowledge this effector is synthesized from inositol trisphosphate by successive phosphorylation. In a search for biosynthetic and degradative intermediates of inositol pentakisphosphate we found only one inositol tetrakisphosphate isomer. Its structure was unambiguously assigned by proton, 13 C‐ and 31 P‐NMR to myo ‐inositol 1,4,5,6‐tetrakisphosphate. As in mammalian cells the major inositol tetrakisphosphate isomer is myo ‐inositol 1,3,4,5‐tetrakisphosphate, there seem to be differences between avian red blood cells and mammalian cells in the routes of inositol polyphosphate formation and/or degradation.