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Molecular cloning and characterization of a full‐length cDNA clone for human plasminogen
Author(s) -
Forsgren Margaretha,
Råden Benny,
Israelsson Marianne,
Larsson Kerstin,
Hedén Lars-Olof
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81501-6
Subject(s) - complementary dna , polyadenylation , coding region , cdna library , microbiology and biotechnology , genetics , nucleic acid sequence , biology , cloning (programming) , dna , gene , messenger rna , computer science , programming language
A human liver cDNA library enriched for full‐length clones was screened for plasminogen cDNA using a synthetic 24‐nucleotide probe derived from a reported partial cDNA sequence. 12 positive clones were identified and one of these was characterized in detail. The 2.7 kb insert contains the complete coding region. At 5 positions, it gives residues different from those reported in a previous amino acid sequence analysis of the protein. The present results show an extra lle at position 65, Gln instead of Glu at positions 53 and 342, Asn at position 88 instead of Asp, and Asp at position 453 rather than Asn. In the 3'‐non‐coding region an extension of 29 bases is found which does not contain any structure compatible with a known polyadenylation signal. Instead, the consensus signal AATAAA is placed at a distance of 46 bases upstream of the poly(A)‐tail.