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The human preproapolipoprotein C‐II gene
Author(s) -
Fojo Silvia S.,
Law Simon W.,
Brewer H.Bryan
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81495-3
Subject(s) - intron , gene , biology , microbiology and biotechnology , genetics , nucleic acid sequence , start codon , nucleic acid , signal peptide , genomic dna , sequence (biology) , peptide sequence , stop codon , nucleotide
The complete nucleic acid sequence of human preproapolipoprotein (apo) C‐II has been determined from 2 apoC‐II clones isolated from 2 different human genomic DNA libraries. The cloned fragments were approx. 14 and 18 kb long, and sequence analysis established that the apoC‐II gene consists of 3338 nucleotides containing 3 intervening sequences of 2391, 167, and 298 bases. The first intron is located within the 5'‐untranslated region of apoC‐II and contains 4 Alu type sequences. The second intron interrupts the codon specifying amino acid —11 of the apoC‐II signal peptide. The last intron, which contains a 38 bp sequence which is repeated 6 times, interrupts the codon specifying for amino acid +44 of the mature apolipoprotein.