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Demonstration of an epidermal growth factor‐dependent 58 kDa phosphoprotein secreted by rat kidney fibroblasts
Author(s) -
Binas Bert,
Grosse Richard
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81484-9
Subject(s) - cycloheximide , epidermal growth factor , phosphoprotein , growth factor , fibroblast growth factor , phosphoserine , chemistry , ammonium chloride , endocrinology , kidney , tgf alpha , transforming growth factor , medicine , biology , microbiology and biotechnology , biochemistry , serine , phosphorylation , protein biosynthesis , receptor , organic chemistry
Epidermal growth factor and 12‐ O ‐tetradecanoylphorbol‐13‐acetate increased the amount of 32 P i found as phosphoserine in a major, hitherto not described 58 kDa phosphoprotein (pp58) secreted by normal rat kidney fibroblasts. Platelet‐derived growth factor, insulin, nerve growth factor and fibroblast growth factor did not affect pp58 while transforming growth factor β decreased the accumulation of radioactivity into pp58. Cycloheximide, actinomycin D and ammonium chloride suppressed the labelling of pp58.