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Glucose regulates preproinsulin messenger RNA levels in a clonal cell line of simian virus 40‐transformed B cells
Author(s) -
Hammonds Peter,
Schofield Paul N.,
Ashcroft Stephen J.H.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81481-3
Subject(s) - messenger rna , northern blot , cell culture , microbiology and biotechnology , biology , rna , insulinoma , gene expression , transcription (linguistics) , gene , insulin , biochemistry , endocrinology , genetics , linguistics , philosophy
In HIT‐T15 cells grown in the absence of glucose, Northern blot analysis of total RNA revealed a major 0.5 kb preproinsulin (ppI) mRNA transcript which co‐migrated with the mature transcript from a human insulinoma. In 4 h tissue cultures, glucose (2–20 mM) stimulated HIT cell ppI mRNA levels in a markedly dose‐dependent manner. Glucose‐stimulated ppI mRNA was (i) inhibited by actinomycin D, suggesting that regulation may be in part transcriptional, and (ii) potentiated by agents known to activate B cell protein kinases. HIT cells represent a unique model for investigating long term regulation of insulin gene expression and biosynthesis.