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The protein receptor for cholerabacteriophage ϕ 149
Author(s) -
Ray Ratna,
Ganguly Uma
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81468-0
Subject(s) - vibrio cholerae , trypsin , sephadex , receptor , chemistry , camp receptor protein , protease activated receptor 2 , protease , chromatography , microbiology and biotechnology , biochemistry , biology , enzyme , bacteria , 5 ht5a receptor , genetics , promoter , gene expression , gene
Choleraphage ϕ 149 receptor activity was found in the outer membrane (OM) protein of Vibrio cholerae 154. Receptor protein for phage ϕ149 was separated from trypsin‐treated OM on a Sephadex G‐100 column. Of the three peaks obtained, phage receptor activity was noted only in peak II. SDS‐PAGE showed that the M r of the protein was 35000. The protein was heat‐labile and protease‐sensitive. The specificity of this protein as choleraphage ϕ 149 receptor was investigated by carrying out a protection experiment by anti‐protein (peak II) rabbit sera.