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Inhibition of taurocholate efflux from rat hepatic canalicular membrane vesicles by glutathione disulfide
Author(s) -
Griffiths James C.,
Sies Helmut,
Meier Peter J.,
Akerboom Theodorus P.M.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81460-6
Subject(s) - glutathione , efflux , chemistry , n ethylmaleimide , vesicle , glutathione disulfide , valinomycin , biochemistry , membrane , enzyme
In right‐side out rat hepatic canalicular membrane vesicles glutathione disulfide (GSSG) inhibited the efflux of taurocholate approx. 70% in the presence or approx. 55% in the absence of a valinomycin‐mediated K + diffusion potential; maximal inhibition occurred at 5 mM GSSG. The inhibition by GSSG was abolished by dithioerythritol. Neither dithioerythritol alone nor GSH inhibited taurocholate efflux. S ‐(2,4‐Dinitrophenyl) glutathione and N ‐ethylmaleimide showed intermediate inhibitory effects.