Premium
Murine erythroleukaemia cells (Friend cells) possess high‐affinity binding sites for erythropoietin
Author(s) -
Mayeux P.,
Billat C.,
Jacquot R.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81442-4
Subject(s) - erythropoietin , microbiology and biotechnology , chemistry , biology , biochemistry , genetics
Murine erythroleukaemia cells represent erythroid precursors blocked near the CFU‐E or proerythroblast stage. In contrast to their non‐leukaemic equivalents, neither their proliferation nor their differentiation seems to be affected by erythropoietin. However, we show in this paper that both uncommitted and committed, benzidine‐positive, cells bind iodinated erythropoietin. The binding is of high affinity ( K d = 490 ± 160 pM) and reversible with a half‐life of the complex of 77 ± 19 min. The number of binding sites is low (300–600 per cell). In contrast the haematopoietic non‐erythroid cell lines HL 60 and L 1210 and the myeloiderythroid human cell line K 562 do not exhibit specific binding. If these binding sites represent true hormone receptors, their presence on a permanent cell line should facilitate erythropoietin receptor purification