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Cloning and sequence analysis of the human brain β‐adrenergic receptor
Author(s) -
Chung Fu-Zon,
Lentes K.-Ulrich,
Gocayne Jeannine,
Fitzgerald Michael,
Robinson Doreen,
Kerlavage Anthony R.,
Fraser Claire M.,
Venter J.Craig
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81436-9
Subject(s) - cdna library , neurotransmitter receptor , biology , receptor , muscarinic acetylcholine receptor , complementary dna , molecular cloning , microbiology and biotechnology , human brain , homology (biology) , gene , cloning (programming) , genetics , neuroscience , computer science , programming language
Two cDNA clones, λ‐CLFV‐108 and λ‐CLFV‐119, encoding for the β‐adrenergic receptor, have been isolated from a human brain stem cDNA library. One human genomic clone, LCV‐517 (20 kb), was characterized by restriction mapping and partial sequencing. The human brain β‐receptor consists of 413 amino acids with a calculated M r of 46 480. The gene contains three potential glucocorticoid receptor‐binding sites. The β‐receptor expressed in human brain was homology with rodent (88%) and avian (52%) β‐receptors and with porcine muscarinic cholinergic receptors (31%), supporting our proposal [(1984) Proc. Natl. Acad. Sci USA 81, 272‐276] that adrenergic and muscarinic cholinergic receptors are structurally related. This represents the first cloning of a neurotransmitter receptor gene from human brain.