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Cholinergic receptors regulate a voltage‐insensitive but Na + ‐dependent calcium influx pathway in salivary acinar cells
Author(s) -
Morris A.P.,
Fuller C.M.,
Gallacher D.V.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81435-7
Subject(s) - cholinergic , calcium , chemistry , receptor , microbiology and biotechnology , biophysics , salivary gland , endocrinology , biology , biochemistry , organic chemistry
The calcium‐sensitive fluorescent probe, quin 2, was employed to investigate the cholinergic receptor regulation of cytosolic free calcium concentration ([Ca 2+ ] i ) in isolated rat submandibular acinar cells. Cholinergic receptor stimulation results in a marked and sustained elevation [Ca 2+ ] i . The major component of this response is shown to be due to activation of a calcium influx pathway. There is no evidence in K + ‐depolarized cells of any voltage‐gated calcium influx. Acetylcholine failed to activate the calcium influx pathway in cells bathed in Na + ‐free solutions. The cholinergic receptors thus regulate a voltage‐insensitive but Na + dependent transport system for calcium entry in submandibular acinar cells.