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Structure determination of the O‐specific polysaccharides from Citrobacter O4‐ and O27‐lipopolysaccharides by methylation analysis and one‐ and two‐dimensional 1 H‐NMR spectroscopy
Author(s) -
Romanowska Elżbieta,
Romanowska Anna,
Dabrowski Janusz,
Hauck Michael
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81431-x
Subject(s) - polysaccharide , nuclear magnetic resonance spectroscopy , methylation , citrobacter , chemistry , spectroscopy , enterobacteriaceae , biochemistry , stereochemistry , escherichia coli , physics , dna , gene , quantum mechanics
Using sugar and methylation analyses, and one‐ and two‐dimensional 1 H‐NMR spectroscopy at 500 MHz it was established that poly‐β‐1,2‐4‐deoxy‐D‐arabinohexopyranose occurs as O‐specific chains of lipopolysaccharides in Citrobacter serotypes O4, O27 and O36. Strong serological cross‐reactivity between these serotypes is in full agreement with the chemical identity of their O‐specific polysaccharides.