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Metabolism of plastid terpenoids: in vitro inhibition of phytoene synthesis by phenethyl pyrophosphate derivatives
Author(s) -
Dogbo Odette,
Bardat Françoise,
Quennemet Joelle,
Camara Bilal
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81339-x
Subject(s) - isopentenyl pyrophosphate , pyrophosphate , phytoene , geranylgeranyl pyrophosphate , terpenoid , chemistry , biochemistry , chromoplast , enzyme , stereochemistry , isomerase , metabolism , biosynthesis , plastid , prenylation , chloroplast , gene
The inhibition of partially purified phytoene synthetase activity from Capsicum annuum chromoplasts was investigated using aminophenethyl pyrophosphate and azidophenethyl pyrophosphate. These compounds were effective inhibitors of phytoene synthesis and kinetic analysis showed that they were competitive with respect to the substrate isopentenyl pyrophosphate. These data were strengthened by the ability of azidophenethyl pyrophosphate to photoinactivate irreversibly the activity of the enzyme complex. These results suggest that the primary targets of these analogs are at the level of isopentenyl pyrophosphate isomerase and geranylgeranyl pyrophosphate synthetase.