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Isolation and characterization of the intact gastrin precursor from a gastrinoma
Author(s) -
Desmond H.,
Pauwels S.,
Varro A.,
Gregory H.,
Young Janice,
Dockray G.J.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81334-0
Subject(s) - gastrinoma , gastrin , isolation (microbiology) , biochemistry , sequence (biology) , chemistry , peptide sequence , complementary dna , stereochemistry , biology , secretion , gene , bioinformatics
Antibodies to the extreme C‐terminal region of human progastrin have been used to monitor the isolation of high‐ M r immunoreactive material in a gastrinoma extract. Microsequence analysis of the product revealed amino acid residues in the first 18 positions corresponding to those predicted from the cDNA sequence for preprogastrin starting at position 22; the sequence and immunochemical data together allow the identification of this material as intact progastrin. Implications for gastrin biosynthesis are discussed.