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Rat liver cytosol oxysterol‐binding protein
Author(s) -
Beseme F.,
Astruc M.E.,
Defay R.,
de Paulet A.Crastes
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81305-4
Subject(s) - cytosol , oxysterol , molecular mass , chemistry , biochemistry , binding protein , plasma protein binding , sedimentation coefficient , dna binding protein , cholesterol , enzyme , transcription factor , gene
A cytosol protein that specifically binds cholesterol derivatives oxygenated on the side chain has been demonstrated in rat liver and transformed HTC cells. This protein, of which the sedimentation coefficient is about 8 S, was saturable and showed a high binding affinity ( K d about 5 × 10 −9 M) for 25‐hydroxycholesterol. Its molecular mass is about 160 kDa. The physicochemical characteristics of this protein were identical whether the model was normal or transformed. This oxysterol‐binding protein differs from the well‐known sterol carrier proteins.