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The developmental regulation of phosphatidylinositol kinase in Dictyostelium discoideum
Author(s) -
Varela Isabel,
Van Lookeren Campagne Michiel M.,
Alvarez Jose F.,
Mato Jose M.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81275-9
Subject(s) - dictyostelium discoideum , phosphatidylinositol , kinase , dictyostelium , mycetozoa , biochemistry , microbiology and biotechnology , biology , chemistry , gene
Phosphatidylinositol kinase was examined in Dictyostelium discoideum since this organism offers molecular and genetic advantages to study the role of phosphatidylinositol metabolism during cell growth and development. D. discoideum homogenates phosphorylated phosphatidylinositol to form phosphatidylinositol 4‐phosphate in a reaction which was found to be linear with time and cell concentration. Optimal activity was obtained in the presence of 1 mM MgCl 2 and pH 7.6 and has an apparent K m for ATP of about 250 μM. Changes in phosphatidylinositol kinase were examined during D. discoideum development. Activity increased about 2‐fold, 4 h after removal of the food source, to decline to almost no activity at late aggregation. During slug formation the activity increased about 15‐fold and remained constant during further development. These results suggest a role for D. discoideum phosphatidylinositol kinase during development.