Premium
Loss of transforming activity of plasmid DNA (pBR322) in E. coli caused by singlet molecular oxygen
Author(s) -
Wefers Heribert,
Schulte-Frohlinde Dietrich,
Sies Helmut
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81272-3
Subject(s) - singlet oxygen , pbr322 , sodium azide , chemistry , dna damage , dna , plasmid , photochemistry , anthracene , methionine , oxygen , escherichia coli , aqueous solution , microbiology and biotechnology , biochemistry , biology , organic chemistry , amino acid , gene
Plasmid DNA pBR322 in aqueous solution was exposed to singlet molecular oxygen ( 1 O 2 ) generated by microwave discharge. DNA damage was detected as loss of transforming activity of pBR322 in E.coli (CMK) dependent on the time of exposure. DNA damage was effectively decreased by singlet‐oxygen quenchers such as sodium azide and methionine. Replacement of water in the incubation buffer by D 2 O led to an increase in DNA damage. 9,10‐Bis(2‐ethylene)anthracene disulfate was used as a chemical trap for 1 O 2 quantitation by HPLC analysis of the endoperoxide formed.