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Mg 2+ ‐ATP induces filament growth from retinal rod outer segments with disrupted plasma membranes
Author(s) -
Parker K.R.,
Schaechter L.E.,
Lewis J.W.,
Zeman K.L.,
Kliger D.S.,
Dratz E.A.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81269-3
Subject(s) - membrane , protein filament , biophysics , plasma , chemistry , colchicine , vanadate , biology , biochemistry , physics , genetics , quantum mechanics
Mg 2+ ‐ATP produces a large decrease in near‐IR light scattering when added to suspensions of rod outer segments (ROS) when the plasma membranes have been disrupted by a gentle dialysis procedure. When this process is studied by light microscopy with video‐enhanced image contrast, the Mg 2+ ‐ATP‐dependent signal is seen to be associated with the formation of filaments which extend only from those ROS lacking plasma membranes. Both the IR light scattering signal and filament growth are inhibited by vanadate and DCCD but not by colchicine, colcemid or cytochalasins.