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A method to exchange alkali light chains on myosin subfragment 1
Author(s) -
Crasnier M.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81268-1
Subject(s) - alkali metal , myosin , immunoglobulin light chain , chemistry , myosin light chain kinase , biophysics , chromatography , biochemistry , organic chemistry , biology , antibody , genetics
Exchange of bound alkali light chains on myosin by free alkali light chains is described. It was found that the yield of hybrid obtained was dependent on the incubation time in 4.7 M NH 4 Cl at pH 9.5. 60% recovery of S1 (A1) from S1(A2) was obtained using only a 2‐fold molar excess of A1 over S1(A2).