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Effect of adrenergic agonists on phosphoinositide breakdown in rat skeletal muscle preparations
Author(s) -
Schadewaldt Peter,
Stapper Norbert J.,
Staib Wolfgang
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81239-5
Subject(s) - prazosin , medicine , endocrinology , phentolamine , inositol phosphate , chemistry , skeletal muscle , agonist , adrenergic receptor , yohimbine , epinephrine , inositol , receptor , biology , antagonist
Adrenergic regulation of phosphoinositide breakdown in rat skeletal muscle was investigated in 30‐min incubations with 10 mM LiCl. In rat hemidiaphragms, prelabelled with D‐ myo ‐[2‐ 3 H]inositol, addition of α‐agonists (epinephrine, norepinephrine, phenylephrine) induced a 5‐8‐fold increase of [ 3 H]inositol monophosphate accumulation. This could be prevented by inclusion of α‐antagonists (phentolamine, prazosin). β‐Agonists and/or β‐antagonists had no effect. Similar experiments with isolated flexor digitorum brevis muscle fibers yielded confirmatory results. Functional integrity of β‐receptor mediated processes was suggested by the β‐agonist‐induced increase of glucose 6‐phosphate in hemidiaphragms and cAMP in fiber preparations. The results indicate that phosphoinositide breakdown in differentiated rat skeletal muscle is, at least in part, under α‐adrenergic control.