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The effect of cadmium ions on 2,3‐bisphosphoglycerate in erythrocytes studied with 31 P NMR
Author(s) -
Arkowitz Robert,
Hoehn-Berlage Mathias,
Gersonde Klaus
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81234-6
Subject(s) - chemistry , cadmium , nmr spectra database , hydrolysis , red blood cell , polyphosphate , aqueous solution , ion , nuclear magnetic resonance spectroscopy , relaxation (psychology) , analytical chemistry (journal) , spectral line , phosphate , chromatography , stereochemistry , biochemistry , organic chemistry , biology , astronomy , neuroscience , physics
The interaction of cadmium ions with human red blood cell (RBC) 2,3‐bisphosphoglycerate (DPG) was studied by 31 P NMR. 31 P NMR spectra and 31 P T 1 and 31 P T 2 relaxation times give evidence for cadmium‐2,3‐bisphosphoglycerate complexation in aqueous solution . 31 P NMR spectra indicate the occurrence of a similar complexation in RBC cryolysates. The lag phase (constant polyphosphate level) prior to DPG hydrolysis in incubated red blood cells is lengthened in the presence of cadmium.