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Ca‐dependent‐chloride and potassium currents in rat Leydig cells
Author(s) -
Duchatelle Pascal,
Joffre Michel
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81232-2
Subject(s) - calcium , potassium , depolarization , biophysics , patch clamp , chemistry , membrane potential , intracellular , voltage clamp , calcium in biology , endocrinology , leydig cell , chloride , current (fluid) , medicine , electrophysiology , biochemistry , biology , hormone , organic chemistry , engineering , electrical engineering , luteinizing hormone
Voltage‐clamped membrane currents have been investigated from whole‐cell patch‐clamp recordings performed on single Leydig cells isolated from the adult rat testis. Two outward membrane currents were evoked by depolarizing voltage steps. A potassium current was recorded in cells dialyzed with low (10 −9 −10 −8 M) calcium media. This current was decreased by TEA (10 mM). A chloride current was recorded in cells dialyzed with high (10 −7 − 10 −6 M) calcium media. This current was decreased by an external exposure to glutamate. Comparison of the currents at low and high internal calcium concentrations suggests that an increase of the intracellular calcium activates a chloride current.