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Botulinum neurotoxin inhibits depolarization‐stimulated protein phosphorylation in pure cholinergic synaptosomes
Author(s) -
Guitart Xavier,
Egea Gustau,
Solsona Carles,
Marsal Jordi
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81220-6
Subject(s) - veratridine , depolarization , acetylcholine , synaptosome , neurotoxin , cholinergic , tetrodotoxin , chemistry , phosphorylation , neurotransmitter , biophysics , biochemistry , biology , pharmacology , neuroscience , membrane , sodium channel , sodium , receptor , organic chemistry
Botulinum neurotoxin, a strong blocker of acetylcholine release at peripheral cholinergic synapses, inhibits depolarization‐stimulated protein phosphorylation in pure cholinergic synaptosomes isolated from the electric organ of Torpedo marmorata . Moreover, tetrodotoxin has the same effect on protein phosphorylation when cholinergic synaptosomes are depolarized by veratridine. Correlation between presynaptic protein phosphorylation and acetylcholine release is suggested by the fact that botulinum neurotoxin blocks specifically neurotransmitter release without affecting membrane depolarization and calcium fluxes in our synaptosomal preparation.