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Size, shape and mass of the oxygen‐evolving photosystem II complex from the thermophilic cyanobacterium Synechococcus sp
Author(s) -
Rögner M.,
Dekker J.P.,
Boekema E.J.,
Witt H.T.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81217-6
Subject(s) - photosystem ii , photosystem i , thermophile , monomer , oxygen evolution , chemistry , cyanobacteria , synechococcus , molecular mass , ionic bonding , crystallography , electron microscope , oxygen evolving complex , photosynthesis , biology , biochemistry , ion , electrochemistry , physics , bacteria , polymer , organic chemistry , optics , electrode , genetics , enzyme
Two different, highly active O 2 ‐evolving photosystem II complexes were purified from the cyanobacterium Synechococcus sp. in the presence of the non‐ionic detergent β‐dodecyl‐D‐maltoside. Both complexes are homogeneous and have molecular masses of approx. 300 and 500 kDa, respectively. By electron microscopy it was found that both complexes have the shape of an elliptical disk, with a thickness of about 6.5 nm and top view dimensions of 10.5 × 15.5 nm for the 300 kDa particle and 18.5 × 15 nm for the 500 kDa particle. It is concluded that the particles represent monomeric and dimeric forms of photosystem II.