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Photolabelling of a mitochondrially encoded subunit of NADH dehydrogenase with [ 3 H]dihydrorotenone
Author(s) -
Earley Fergus G.P.,
Patel Salil D.,
Ragan C.Ian,
Attardi G.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81200-0
Subject(s) - nadh dehydrogenase , antiserum , protein subunit , gene product , immunoprecipitation , microbiology and biotechnology , biochemistry , gene , enzyme , labelling , chemistry , biology , antibody , gene expression , genetics
Mitochondrial NADH dehydrogenase from bovine heart was photolabelled with the inhibitor [ 3 H]dihydrorotenone. A constituent of the hydrophobic domain of the enzyme of M r 33 000 was the major site of labelling. The identity of this protein with the mitochondrially encoded ND‐1 gene product was established by immunoblotting and immunoprecipitation with an antiserum raised to the expected C‐terminal sequence of the human ND‐1 gene product.