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Deletion analysis of the c‐Ha‐ ras oncogene promoter
Author(s) -
Trimble William S.,
Hozumi Nobumichi
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81192-4
Subject(s) - oncogene , microbiology and biotechnology , chemistry , biology , genetics , gene , cell cycle
Using transient assays of chloramphenicol acetyltransferase expression in CV‐1 cells, we have localized the human c‐Ha‐ ras oncogene promoter to a 550 bp fragment located about 1 kb upstream from the ras coding sequence. Deletion analysis has revealed that a 100 nucleotide region 200 base pairs upstream of the putative initiation sites for transcription is essential for high levels of expression. Within this sequence lie two Spl binding sites and a consensus CCAAT box.