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Infection of tobacco protoplasts with in vitro transcribed tobacco mosaic virus RNA using an improved electroporation method
Author(s) -
Watanabe Yuichiro,
Meshi Tetsuo,
Okada Yoshimi
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81191-2
Subject(s) - tobacco mosaic virus , electroporation , biology , complementary dna , rna , in vitro , virology , reverse transcriptase , virus , microbiology and biotechnology , gene , genetics
We previously established an in vitro transcription system which can produce infectious tobacco mosaic virus (TMV) RNAs from cDNA templates [(1986) Proc. Natl. Acad. Sci. USA 83, 5043‐5047]. Here we report a useful system for the infection of tobacco protoplasts with a small amount of infectious RNAs, like in vitro transcripts from cDNA templates, involving an improved electroporation method. This system will facilitate the elucidation of the functions of the genomic RNA and its coding proteins using in vitro manipulated TMV‐RNAs.