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Glucose transport in human erythrocytes measured using 13 C NMR spin transfer
Author(s) -
Kuchel Philip W.,
Chapman Bogdan E.,
Potts Jennifer R.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81180-8
Subject(s) - chemistry , glucose transporter , spin (aerodynamics) , magnetization transfer , nuclear magnetic resonance , physics , biology , medicine , insulin , thermodynamics , radiology , magnetic resonance imaging , endocrinology
We present the results of a new NMR‐based procedure for measuring the fast transmembrane exchange of D‐[1‐ 13 C]glucose in human erythrocytes. The method relies on different rates of exchange between the α‐ and β‐anomers of glucose inside and outside the cells; the rate outside the cells is greatly increased by the addition of mutarotase to the suspension. Theory is developed to describe nuclear‐spin transfer in the present system and is used to analyse the data to yield estimates of transmembrane‐exchange rate constants and their statistical uncertainties. For a total glucose concentration of 25.5 mmol/1 at 40°C the first order efflux rate constants for the α‐ and β‐anomers were 1.20±0.40 s −1 and 0.71±0.30 s −1 , respectively.