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Heterogeneity of the amino acid sequence of phytochrome from etiolated oat seedlings
Author(s) -
Grimm Rudolf,
Lottspeich Friedrich,
Rüdiger Wolfhart
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81160-2
Subject(s) - etiolation , valine , phytochrome , methionine , biochemistry , amino acid , subtilisin , biology , gene , chemistry , botany , enzyme , red light
Phytochrome from etiolated oat seedlings was digested with subtilisin. A 16 kDa fragment was isolated and investigated by microsequencing. Its amino‐terminal sequence SLPGGSMEV/ML revealed a heterogeneity (valine and methionine) at position 9. This proves expression of several phytochrome genes on the protein level. One can conclude from the ratio valine/methionine that the isophytochrome derived from AP5 (containing methionine) is a major gene product besides isophytochromes derived from genes AP4 and AP3 (containing valine).