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A ribosome‐associated inhibitor of in vitro nonsense suppression in [ psi − ] strains of yeast
Author(s) -
Tuite Mick F.,
Cox Brain S.,
McLaughlin Calvin S.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81158-4
Subject(s) - yeast , strain (injury) , in vitro , lysis , translation (biology) , nonsense , ribosome , chemistry , eukaryote , transfer rna , microbiology and biotechnology , biology , biochemistry , rna , messenger rna , gene , genome , anatomy
All classes of tRNA‐mediated nonsense suppression are much more efficient in yeast cell‐free lysates prepared from a [ psi + ] strain than in those prepared from an isogenic [ psi − ] strain. Mixed [ psi + ]/[ psi − ] lysates do not support efficient suppression. Fractionation of the [ psi − ] lysate demonstrated the presence of an inhibitor of in vitro suppression that is loosely associated with the 80 S ribosome. The data indicate that the inhibitor is a factor involved in the termination of translation in this simple eukaryote.