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A 31 P MAS NMR study of cytidine 2′‐phosphate bound to ribonuclease A in the crystalline state
Author(s) -
Dobson Christopher M.,
Lian Lu-Yun
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81154-7
Subject(s) - cytidine , ribonuclease , chemistry , phosphate , crystallography , rna , biochemistry , enzyme , gene
31 P CP/MAS spectra have been obtained from 2′‐CMP bound to ribonuclease A in the crystalline state. The chemical shift value is closely similar to that found in solution NMR studies under similar conditions, and corresponds to that of the dianionic state of the free compound. It is suggested that the NMR approach may be of general applicability for the comparison of the binding properties of small molecules to proteins in crystals and solution.