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Generation of OH radical during enzymatic reduction of 9,10‐anthraquinone‐2‐sulphonate Can semiquinone decompose hydrogen peroxide?
Author(s) -
Sushkov D.G.,
Gritsan N.P.,
Weiner L.M.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81146-8
Subject(s) - chemistry , semiquinone , radical , hydrogen peroxide , photochemistry , spin trapping , anthraquinone , flash photolysis , hydroxyl radical , reductase , oxygen , enzyme , redox , inorganic chemistry , kinetics , organic chemistry , reaction rate constant , physics , quantum mechanics
Spin‐trapping experiments have shown that 9, 10‐anthraquinone‐2‐sulphonate (AQS) can be reduced enzymatically with NADPH‐cytochrome P‐450 reductase (FP) producing OH radicals. It has been revealed for the first time that the radical anion of AQS cannot decompose H 2 O 2 as indicated by flash‐photolysis data. From the experimental results obtained it follows that an enzyme system containing NADPH, AQS and FP produces both accumulation of H 2 O 2 from molecular oxygen and reduction of Fe 3+ to Fe 2+ which decomposes H 2 O 2 catalytically via a Fenton reaction.