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Loss of inducible photorepair in a frog cell line hypersensitive to solar UV light
Author(s) -
Chao Chuck C.-K.,
Lin-Chao Sue
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)81145-6
Subject(s) - mutant , electron paramagnetic resonance , cell culture , wild type , microbiology and biotechnology , cell , biology , chemistry , biochemistry , gene , genetics , physics , nuclear magnetic resonance
The induction of enzymatic photorepair (EPR) in ICR 2A frog cells and a derived mutant cell line DRP36 hypersensitive to solar UV was studied. Using clonogenic assays, when induced wild‐type cells demonstrated an 8‐fold increase of EPR the mutant cells displayed a near‐background level of inducible EPR. The constitutive EPR in mutant cells, however, was the same as in wild‐type cells. A mixed culture of ICR 2A and DRP36 cells showed an intermediate inducible EPR depending upon the cell ratio. Inducible EPR was also detected at the DNA level in wild‐type cells, but not in mutant cells.